CenH3-Independent Kinetochore Assembly in Lepidoptera Requires CCAN, Including CENP-T
نویسندگان
چکیده
منابع مشابه
The CCAN recruits CENP-A to the centromere and forms the structural core for kinetochore assembly
CENP-A acts as an important epigenetic marker for kinetochore specification. However, the mechanisms by which CENP-A is incorporated into centromeres and the structural basis for kinetochore formation downstream of CENP-A remain unclear. Here, we used a unique chromosome-engineering system in which kinetochore proteins are targeted to a noncentromeric site after the endogenous centromere is con...
متن کاملMolecular basis of outer kinetochore assembly on CENP-T
Stable kinetochore-microtubule attachment is essential for cell division. It requires recruitment of outer kinetochore microtubule binders by centromere proteins C and T (CENP-C and CENP-T). To study the molecular requirements of kinetochore formation, we reconstituted the binding of the MIS12 and NDC80 outer kinetochore subcomplexes to CENP-C and CENP-T. Whereas CENP-C recruits a single MIS12:...
متن کاملCENP-T provides a structural platform for outer kinetochore assembly.
The kinetochore forms a dynamic interface with microtubules from the mitotic spindle during mitosis. The Ndc80 complex acts as the key microtubule-binding complex at kinetochores. However, it is unclear how the Ndc80 complex associates with the inner kinetochore proteins that assemble upon centromeric chromatin. Here, based on a high-resolution structural analysis, we demonstrate that the N-ter...
متن کاملThe distinct functions of CENP-C and CENP-T/W in centromere propagation and function in Xenopus egg extracts
The centromere is the chromosomal region in which the kinetochore is assembled to orchestrate chromosome segregation. It is defined by the presence of a histone H3 variant called Centromere Protein A (CENP-A) or CenH3. Propagation of centromere identity entails deposition of new CENP-A upon exit from mitosis in vertebrate cells. A group of 16 proteins that co-immunoprecipitate with CENP-A, the ...
متن کاملCCAN Makes Multiple Contacts with Centromeric DNA to Provide Distinct Pathways to the Outer Kinetochore
Kinetochore specification and assembly requires the targeted deposition of specialized nucleosomes containing the histone H3 variant CENP-A at centromeres. However, CENP-A is not sufficient to drive full-kinetochore assembly, and it is not clear how centromeric chromatin is established. Here, we identify CENP-W as a component of the DNA-proximal constitutive centromere-associated network (CCAN)...
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ژورنال
عنوان ژورنال: Current Biology
سال: 2020
ISSN: 0960-9822
DOI: 10.1016/j.cub.2019.12.014